The dotLab® Sensor

The dot®- Covalent Sensor

Providing Researchers with a new tool for immunoassay design and interaction experiments

 

  • Stable covalent coupling of affinity reagents
  • Directly link capture species covalently, excellent alternative when commercially biotinylated antibodies are not available
  • Eliminate the need for offline conjugation
  • Reduce protein sample requirement 50-fold*
  • Only a few microliters of sample required for analysis
  • Compatible with regeneration for increased throughput or rapid comparative experiments
  • Lidless format makes sample recovery possible for offline Mass Spectrometry analysis
  • Inexpensive tubing sets permit single or reuse

dotLab Sensor

The dot®- Avidin Sensor

  • Disposable, Cost Effective
  • Only a few microliters of sample required for analysis
  • Utilizes the robust biotin-avidin interaction to rapidly immobilize capture reagents
  • Compatible with dotReady reagents or choose from thousands of readily available commercial biotinylated antibodies
  • Directly convert plate based avidin or streptavidin immunoassays to the dotLab real-time format

Diffractive optics technology (dot®)

  • Analyte detection can be performed across a broad dynamic range allowing the measurement of picomolar and micromolar target levels in the same sample
  • Real-time observation of binding interactions allow immediate understanding of complex assays
  • Ideal for complex samples:  The detection beam never passes through the flow channel so biological samples such as serum, plasma or crude cell lysates can all be used and require only minimal sample preparation
  • Reduced impact of non-specific binding
  • Allows direct measurement in complex media
  • No requirement for separate reference channel allows broad range of experimental formats
  • Self referencing remove flow artifacts and allows vigorous mixing
  • Significantly reduces sample volume requirements

 

SensorAssay Spots

  • Core to the sensor technology is the use of a diffraction grating composed of affinity reagents such as antibodies.
  • This grating is made from a repetitive sequence of lines and generates a fixed diffraction pattern when illuminated.
  • This grating is provided pre-pattered on the lower surface of 10 µL flow channel to allow attachment of capture reagent or target in a disposable, plastic sensor.
  • Each dotLab Sensor contains multiples assay spots located along its linear flow channel allowing for replicate or multiplex analysis within a single sample
  • The dotLab System introduces samples and assay reagents into the sensor using an automated sampling system and high-precision fluidic controller
  • An integral prism allows continuous interrogation of the pattern from below to create the diffraction signal without interference from the sample matrix
  • As molecules bind to the sensor surface, the real-time signal responds in proportion to molecular size and concentration
  • Several attachment chemistries allow the user flexibility in coupling their own content to the sensors for experiments.

*A typical micro biotinylation kit requires at least 50 microgram of protein to successfully label with a biotin. The Amine Reactive Sensor requires only 1 microgram of protein to couple directly onto the surface. A standard reaction on the Amine Reactive Sensor uses 100 microliters of protein at a concentration of 10 ug/mL, which equals 1 microgram of total protein needed. This is 50 fold less than the total protein needed for the biotinylation process.