Technology

Overview

Flow-through technologies for simplifying multiplex biomarker testing.

At Axela, our goal is to simplify multiplex assays for clinical and diagnostic applications through automation and customization. Our flow-through platforms use the robust technologies of flow-through microarrays and diffractive optics sensors to accelerate the biomolecular interactions that occur during an assay, thereby reducing result times while providing the high sensitivity and reproducibility required in biomarker testing and clinical diagnostics. A summary of the capabilities of both platforms is given below:

	Ziplex® System	Diffractive Optics
Analytes	Proteins or nucleic acids	Proteins
Assay consumable	Flow-Thru Microarray	Diffractive Optics Sensor
Detection system	End point (Chemiluminescence)	Real time (Diffractive optics)
Multiplex	≤150 (proteins or nucleic acids) or ≤12 (proteins with panelPlus™)	1-12 (with panelPlus™) 1-32 with Sensor Disk
Custom multiplex	Yes, with panelPlus™	Yes, with panelPlus™
Automated assays	Yes, only sample dilution is required for protein assays, standard RNA extraction and labelling for nucleic acids	Yes, only sample dilution is required!
Total assay run time	30 (proteins) – 180 (nucleic acids) minutes	10 – 30 minutes
Samples per assay	8	1-8 (instrument model dependant)
Total hands on instrument time per run	≤20 minutes	≤10 minutes
Sample Type	Serum, FFPE, swabs, lysates	Serum, plasma, urine, stool, nasal swab, crude extracts, lysates or other complex matrices
Sample volume	≤ 5 uL	≤ 5 uL
Sensitivity	Low pg/mL for proteins; femtomolar levels for oligos	Low pg/mL
Dynamic range	4 logs	7 logs
% CV	≤ 5-15%	≤ 5-15%
Applications	Targeted Discovery Biomarker Verification Panel Optimization Panel Validation Routine Customized Assays Future Central Laboratory Testing	Assay Development Panel Optimization Panel Validation Routine Customized Assays Future Point of Use Applications